Resources

Resources

Antibodies

Testing Rabbit Bleeds by ELISA
Coupling Peptides to Resin for Affinity Purification
Preparation of Peptide
Purification of Anti-Peptide Antibody
On-resin IgG labeling with Alexa flour NHS esters
Chromosomes and Kinetochores

Sperm Chromatin Assembly in CSF or Interphase HSS
CHO Chromosome Prep
Fixing and Pelleting Chromatin
General Techniques

In Gel Tryptic Digest
Flourescence Procedure for the Actin and Tubulin Cytoskeleton in Fixed Cells
Microinjection of Single Cells in Culture
Microtubules

Negative Stain Electron Microscopy of Microtubules
Tubulin Polymerization with GTP
Tubulin Basics
Recycling Tubulin
Preparation of Segmented and Polarity Marked Microtubules
Microtubule Spindowns from Extracts
Microtubule Spindowns for Visual Analysis
Large Scale Tubulin Preparation
Labeling Tubulin and Quantifying Labeling Stoichiometry
Flow Cell Assays with Microtubules
CHO Centrosome Prep
Xenopus Egg Extract

HSS Immunodepletion
Crude Extract prep for Spindle Assembly
Crude Extract Immunodepletion